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Title 

Development of selection marker-free transgenic potato plants with enhanced tolerance to oxidative stress

Authors 

R AhmadYun Hee KimMyoung Duck KimM N PhungW I ChungHaeng Soon LeeSang Soo KwakSuk Yoon Kwon

Publisher 

The Botanical Society of Korea

Issue Date 

2008

Citation 

Journal of Plant Biology, vol. 51, no. 6, pp. 401-407

Keywords 

genetic transformationmarker-free transgenic plantsoxidative stresspotato

Abstract 

A binary vector devoid of a plant selection-marker gene (designated as pSSA-F) was constructed to overcome bio-safety concerns about genetically modified plants. This vector carried chloroplast-targeted superoxide dismutase (SOD) and ascorbate peroxidase (APX) genes under the control of an oxidative stress-inducible(SWPA2) promoter, and was utilized to transform potato (Solanum tuberosum L.). Integration of these foreign genes into transgenic plants was primarily performed via PCR with genomic DNA. Twelve marker-free transgenic lines were obtained by inoculating stem explants. The maximum transformation efficiency was 6.25% and averaged 2.2%. Successful integration of the SOD and APX genes rendered transgenic plants tolerant to methyl viologen-mediated oxidative stress at the leaf-disc and whole-plant levels. Our findings suggest that this technique for developing selection marker-free transgenic plants is feasible and can be employed with other crop species.

ISSN 

1226-9239

Link 

http://dx.doi.org/10.1007/BF03036060

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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