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Title 

A two-component regulatory system involved in clavulanic acid production

Authors 

H N JnawaliT J OhK LiouByoung Chul ParkJ K Sohng

Publisher 

Nature Publishing Group

Issue Date 

2008

Citation 

Journal of Antibiotics, vol. 61, no. 11, pp. 651-659

Keywords 

β-lactam antibioticsclavulanic acidhistidine kinaseresponse regulatorstreptomyces clavuligerustwo-component regulatory systemamino acid sequencebacterial genemolecular cloning

Abstract 

A pair of genes encoding the bacterial two-component regulatory system, orf22 and orf23, was found next to the clavulanic acid gene cluster of Streptomyces clavuligerus NRRL3585. Orf23 was deleted for the construction of S. clavuligerus I δorf2 3. Although growth and morphological analyses showed no differences between S. clavuligerus/δorf23 and wild-type, the production of clavulanic acid in S. clavuligerus / δorf23 was found to be decreased. In addition, the co-overexpression of orf22/orf23 in wild-type resulted in an enhanced 1.49-fold production of clavulanic acid, and the complementation of orf22/orf23 in S. clavuligerus / δorf23 restored clavulanic acid production about 80% as normal levels. These results demonstrate that the orf22/orf23 two-component regulatory system participates as a positive regulator of the biosynthesis of clavulanic acid and increased levels of orf22/orf23 can contribute to enhanced production of clavulanic acid in S. clavuligerus. ⓒ Japan Antibiotics Research Association.

ISSN 

0021-8820

Link 

http://dx.doi.org/10.1038/ja.2008.92

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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