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Title 

Multiple-layer substrate zymography for detection of several enzymes in a single sodium dodecyl sulfate gel

Authors 

Nack-Shick ChoiBo-Hye KimC S ParkYun-Jon HanHye-Won LeeJ H ChoiSeung Goo LeeJae Jun Song

Publisher 

Elsevier

Issue Date 

2009

Citation 

Analytical Biochemistry, vol. 386, no. 1, pp. 121-122

Keywords 

bacterial proteinscellulaseelectrophoresis, polyacrylamide gelenzymeslipasepeptide hydrolasesresearch designbacillus sp.paenibacillus sp.staphylococcus sp.

Abstract 

We have developed a system to detect three hydrolytic enzymes (cellulase, lipase, and protease) using a single sodium dodecyl sulfate (SDS) gel and an electrotransfer system. After electrophoresis, proteins in the gel were transferred to three sandwiched substrate gels containing glycerol tributyrate, azo-carboxymethyl cellulose (Azo-CMC), and fibrin for detection of cellulase, lipase, and protease, respectively. We show that three cellulases (from a Paenibacillus sp. and two Bacillus sp. strains), one lipase (from a Staphylococcus sp.), and two proteases (from two Bacillus sp. strains) can be detected simultaneously with our zymogram system.

ISSN 

0003-2697

Link 

http://dx.doi.org/10.1016/j.ab.2008.11.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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