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Title 

The effects of acrylamide on brain creatine kinase: Inhibition kinetics and computational docking simulation

Authors 

Z R LuH C ZouSung Jin ParkDae Ui ParkL ShiSang Ho OhY D ParkJong Hwa ParkF Zou

Publisher 

Elsevier

Issue Date 

2009

Citation 

International Journal of Biological Macromolecules, vol. 44, no. 2, pp. 128-132

Keywords 

acrylamidebrain creatine kinaseinhibition kineticscreatine kinase BBcomputer simulationmolecular docking

Abstract 

The occurrence of acrylamide is frequently observed in processed foods. Therefore, the harmful effects of acrylamide on metabolic enzymes are important to understand. We studied the inhibitory effects of acrylamide on the brain creatine kinase (CK-BB). We found that CK-BB was kinetically inactivated by acrylamide accompanied by the disruption of the hydrophobic surface. Acrylamide mainly interacted with the thiol (-SH) residue of CK-BB and resulted in alkylation. A computational docking simulation supported that acrylamide directly bound to the active site of CK-BB where cysteine and glycine residues interacted mainly. The inhibition kinetics combined with computational prediction can be useful in order to have insights into the mechanisms regarding environmentally hazardous factors at the molecular level.

ISSN 

0141-8130

Link 

http://dx.doi.org/10.1016/j.ijbiomac.2008.11.003

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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