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Title 

Inhibition of human cervical carcinoma growth by cytokine-induced killer cells in nude mouse xenograft model

Authors 

Hwan Mook KimJ LimJong Soon KangSong Kyu ParkKiho LeeJ Y KImY J KimJ T HongY KimS B Han

Publisher 

Elsevier

Issue Date 

2009

Citation 

International Immunopharmacology, vol. 9, no. 3, pp. 375-380

Keywords 

adoptive immunotherapycytokine-induced killer cellsKB-3-1 cervical cancercytokineanimal modelcancer growthcell destructioncell populationcellular immunityeffector cell

Abstract 

Cervical cancer is a major cause of cancer mortality in women worldwide and is an important public health problem for adult women in developing countries. Despite aggressive treatment with surgery and chemoradiation, the outcomes for cervical cancer patients remain poor. In this study, the antitumor activity of cytokine-induced killer (CIK) cells against human cervical cancer was evaluated in vitro and in vivo. Human peripheral blood mononuclear cells were cultured with IL-2-containing medium in anti-CD3 antibody-coated flasks for 5 days, followed by incubation in IL-2-containing medium for 9 days. The resulting populations of CIK cells comprised 95% CD3+, 3% CD3-CD56+, 35% CD3+CD56+, 11% CD4+, < 1% CD4+CD56+, 80% CD8+, and 25% CD8+CD56+. At an effector-target cell ratio of 100:1, CIK cells destroyed 56% of KB-3-1 human cervical cancer cells, as measured by the 51Cr-release assay. In addition, CIK cells at doses of 3 and 10 million cells per mouse inhibited 34% and 57% of KB-3-1 tumor growth in nude mouse xenograft assays, respectively. This study suggests that CIK cells may be used as an adoptive immunotherapy for cervical cancer patients.

ISSN 

1567-5769

Link 

http://dx.doi.org/10.1016/j.intimp.2008.12.001

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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