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Title 

Prostacyclin stimulates embryonic development via regulation of the cAMP response element-binding protein-cyclo-oxygenase-2 signalling pathway in cattle

Authors 

Bong Seok SongJi Su KimC H KimY M HanD S LeeKyung Kwang LeeDeog Bon Koo

Publisher 

CSIRO Publishing

Issue Date 

2009

Citation 

Reproduction Fertility and Development, vol. 21, no. 3, pp. 400-407

Keywords 

iloprostcyclic AMP responsive element binding proteincyclooxygenase 2embryo developmentprotein expression

Abstract 

Prostacyclin (PGI2) in oviducal fluid is synthesised from arachidonic acid by cyclo-oxygenase (COX) and prostacyclin synthetase and enhances the implantation and live birth potential of mouse embryos. In the present study, we investigated the developmental competence of bovine embryos by examining the effects of the PGI2 analogue iloprost on blastocyst development, quality and COX-2 expression during IVF and somatic cell nuclear transfer (SCNT). Bovine IVF and SCNT embryos were cultured in CR1-aa medium supplemented with 0.3% bovine serum albumin in either the presence or absence of 1 μm iloprost at 38.5°C and 5% CO2. After 3 days of culture, cleaved embryos were cultured for 4 days in the same medium supplemented with 10% fetal bovine serum. For both IVF and SCNT embryos, iloprost improved the blastocyst developmental rate and cell numbers. In the presence of iloprost, the proportion of expanded blastocysts was significantly higher among the IVF embryos and fewer apoptotic cell nuclei were observed. Expression of COX-2 mRNA and protein, evaluated using real-time polymerase chain reaction and immunoblotting, respectively, was increased in the presence of iloprost. These results suggest that PGI2 improves the developmental competence of embryos via regulation of the cAMP response element-binding protein-COX-2 signalling pathway in cattle.

ISSN 

1031-3613

Link 

http://dx.doi.org/10.1071/RD08180

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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