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Title 

Obovatol isolated from Magnolia obovata enhances pentobarbital-induced sleeping time: Possible involvement of GABA(A) receptors/chloride channel activation

 

오보바톨의 수면 개선 효과

Authors 

H MaY J JoY MaJ T HongByoung-Mog KwonK W Oh

Publisher 

Elsevier

Issue Date 

2009

Citation 

Phytomedicine, vol. 16, no. 4, pp. 308-313

Keywords 

chloride influxGABAA receptorsglutamic acid decarboxylase (GAD)magnolia obovataobovatolpentobarbitalsleepsleep time

Abstract 

This study aimed to investigate the effects of obovatol isolated from Magnolia obovata on pentobarbital-induced sleeping behaviors and to determine whether these effects were mediated by GABAA receptors/chloride channel activation, using a western blot technique and Cl- sensitive fluorescence probe. GABAA receptors subunits expression and chloride influx were investigated in cultured cerebellar granule cells. Obovatol (0.05, 0.1, and 0.2 mg/kg) prolonged the sleeping time induced by pentobarbital (42 mg/kg). In addition, obovatol (20 and 50 μM) significantly increased Cl- influx in the primary cultured cerebellar granule cells. Moreover, obovatol increased the expression of GABAA receptor α-, β-, and γ-subunits. However, it had no effect on the abundance of the expression of glutamic acid decarboxylase (GAD), suggesting that obovatol might not activate GAD. These results suggest that obovatol potentiates pentobarbital-induced sleeping time through the GABAA receptors/chloride channel activation.

ISSN 

0944-7113

Link 

http://dx.doi.org/10.1016/j.phymed.2008.12.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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