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Title 

Molecular characterization of the sweet potato peroxidase SWPA4 promoter which responds to abiotic stresses and pathogen infection

 

환경스트레스와 병원균 감염에 반응하는 고구마 퍼옥시다제 SWPA4 promoter의 분자특성

Authors 

Sun Hwa RyuYun Hee KimC Y KimS Y ParkSuk Yoon KwonHaeng Soon LeeSang Soo Kwak

Publisher 

Wiley-Blackwell

Issue Date 

2009

Citation 

Physiologia Plantarum, vol. 135, no. 4, pp. 390-399

Keywords 

cells, culturedchromosome walkinggene expression regulation, planthydrogen peroxideipomoea batataseffectsperoxidasesplant proteinsplants, genetically modifiedpromoter regions, genetic

Abstract 

Previously, the swpa4 peroxidase gene has been shown to be inducible by a variety of abiotic stresses and pathogenic infections in sweet potato (Ipomoea batatas). To elucidate its regulatory mechanism at the transcriptional level under various stress conditions, we isolated and characterized the promoter region (2374 bp) of swpa4 (referred to as SWPA4). We performed a transient expression assay in tobacco protoplasts with deletions from the 5′-end of SWPA4 promoter fused to the β-glucuronidase (GUS) reporter gene. The -1408 and -374 bp deletions relative to the transcription start site (+1) showed 8 and 4.5 times higher GUS expression than the cauliflower mosaic virus 35S promoter, respectively. In addition, transgenic tobacco plants expressing GUS under the control of -2374, -1408 or -374 bp region of SWPA4 promoter were generated and studied in various tissues under abiotic stresses and pathogen infection. Gel mobility shift assays revealed that nuclear proteins from sweet potato cultured cells specifically interacted with 60-bp fragment (-178/-118) in -374 bp promoter region. In silico analysis indicated that four kinds of cis-acting regulatory sequences, reactive oxygen species-related element activator protein 1 (AP1), CCAAT/enhancer-binding protein alpha element, ethylene-responsive element (ERE) and heat-shock element, are present in the -60 bp region (-178/-118), suggesting that the -60 bp region might be associated with stress inducibility of the SWPA4 promoter.

ISSN 

0031-9317

Link 

http://dx.doi.org/10.1111/j.1399-3054.2008.01197.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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