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Title 

Pyrrolidine dithiocarbamate-induced activation of ERK and increased expression of c-fos in mouse embryonic stem cells

Authors 

Y E KimJ A ParkKi Hoan NamH J KwonY Lee

Publisher 

Korean Society for Biochemistry and Molecular Biology

Issue Date 

2009

Citation 

BMB Reports, vol. 42, no. 3, pp. 148-153

Abstract 

Pyrrolidine dithiocarbamate (PDTC) is a stable anti-oxidant or pro-oxidant, depending on the situation, and it is widely used to inhibit the activation of NF-kappaB. We recently reported that PDTC activates the MIP-2 gene in a NF-kappaB-independent and c-Jun-dependent manner in macrophage cells. In this work, we found that PDTC activates signal transduction pathways in mouse ES cells. Among the three different mitogen-activated protein kinase (MAPK) pathways, including the extracellular-signal-regulated kinase (ERK), p38 MAP kinase, and stress-activated protein kinase (SAPK)/Jun N-terminal kinase (JNK) pathways, only the ERK pathway was significantly activated in mouse ES cells after stimulation with PDTC. Additionally, we observed a synergistic activation of ERK and induction of c-Fos after stimulation with PDTC in the presence of mouse embryonic fibroblast (MEF) conditioned medium. In contrast, another NF-kappaB inhibitor, BMS-345541, did not activate the MAP kinase pathways or induce expression of c-Fos. These results suggest that changes in the presence of the NF-kappaB inhibitor PDTC should be carefully considered when it used with mouse ES cells.

ISSN 

1976-6696

Link 

http://dx.doi.org/10.5483/BMBRep.2009.42.3.148

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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