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Title 

Rengyolone inhibits apoptosis via etoposide-induced caspase downregulation

Authors 

Jin Hee KimC H Lee

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2009

Citation 

Journal of Microbiology and Biotechnology, vol. 19, no. 3, pp. 286-290

Keywords 

apoptosisrengyolone

Abstract 

In the course of screening for substances inhibiting apoptosis of U937 human leukemia cells induced by etoposide (10 μg/ml), Forsythiae fructus, which showed a high level of inhibition, was selected. The regulating compounds were purified from the ethyl acetate extract by silica gel column chromatography and HPLC. The active substance was purified and identified as rengyolone by spectroscopic methods. This compound showed inhibitory activity on caspase-3 induction, a major protease of the apoptosis cascade, with an IC50 value of 38.96 μM after 8 h of etoposide treatment in U937 cells. The expression level of caspase-3 and poly(ADP-ribose) polymerase (PARP) were dose-dependently inhibited by the compound, suggesting that rengyolone inhibits etoposide-induced apoptosis via downregulation of caspases.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.0804.253

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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