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Title 

A framework for molecular genetic assessment of a transgenic watermelon rootstock line

 

형질전환 수박 접목 계통의 분자유전학적 평가를 위한 기본 구조

Authors 

Eun Soo YoukIn Soon PackYu Jin KimWoon Kee YoonChang-Gi KimStephen Beungtae RueC H HarnSoon Chun JeongHwan Mook Kim

Publisher 

Elsevier

Issue Date 

2009

Citation 

Plant Science, vol. 176, no. 6, pp. 805-811

Keywords 

CGMMV-CProotstocksatsciontransgenicwatermelon

Abstract 

Transgenic plants over-expressing virus coat protein genes have attracted particular interest from researchers recently, due primarily to their tolerance to viral infection. The transgenic watermelon rootstock analyzed in this study contains the introduced cucumber green mottle mosaic virus coat protein (CGMMV-CP) gene. The primary objective of this study was to determine the copy number, integration site, and expression level of the transgene element, in order to establish a scientific framework for the molecular genetic assessment of transgenic plant rootstocks. The results of our Southern blot analysis indicated that a single copy of the CGMMV-CP gene was inserted into the genome of a transgenic watermelon rootstock. We also identified the genomic sequences flanking the integration site of the transgene via inverse PCR analysis. In an effort to find a sequence usable as an internal positive control for the screening of the transgenic watermelons, we determined that the Sat gene appears as one copy within their genomes and is watermelon-specific. The information from the integrated site and the internal positive control sequence was utilized to establish a new event-specific PCR-based detection method. The expression of both CGMMV-CP mRNA and protein was detected in the transgenic watermelon rootstocks but not in watermelon scions, thereby suggesting that the tissues of the watermelon scions are free of the introduced gene products.

ISSN 

0168-9452

Link 

http://dx.doi.org/10.1016/j.plantsci.2009.03.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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