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Title 

Selection of transgenic sweetpotato plants expressing 2-Cys peroxiredoxin with enhanced tolerance to oxidative stress

 

Peroxiredoxin 유전자 발현 산화스트레스 내성 형질전환 고구마의 선발

Authors 

Myoung Duck KimKyoung-Sil YangSuk Yoon KwonS Y LeeSang Soo KwakHaeng Soon Lee

Publisher 

Korea Society of Plant Biotechnology

Issue Date 

2009

Citation 

Journal of Plant Biotechnology, vol. 36, no. 1, pp. 75-80

Abstract 

In order to develop transgenic sweetpotato plants [Ipomoea batatas (L.) Lam. cv. Yulmi] with enhanced tolerance to oxidative stress, we constructed transformation vectors expressing 2-Cys peroxiredoxin (Prx) gene under the control of the stress-inducible SWPA2 or enhanced 35S promoter (named as SP or EP). Transgenic sweetpotato plants were attempted to generate from embryogenic calli using an Agrobacterium-mediated transformation system. Embryogenic calli gave rise to somatic embryos and then converted into plantlets on MS medium containing 100 mg/L kanamycin. Transgenic plants were regenerated in the same medium. Southern blot analysis confirmed that the Prx gene was inserted into the genome of the plants. To further study we selected the transgenic plant lines with enhanced tolerance against methyl viologen (MV). When sweetpotato leaf discs were subjected to methyl MV at 20 μM, transgenic plants showed about 40% higher tolerance than non-transgenic or empty vector-transformed plants.

ISSN 

1229-2818

Link 

http://dx.doi.org/10.5010/JPB.2009.36.1.075

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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