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Title 

Involvement of PTP-RQ in differentiation during adipogenesis of human mesenchymal stem cells

Authors 

Hyeyun JungWon Kon KimD H KimYeesook ChoSeung Jun KimSung Goo ParkByoung Chul ParkH M LimKwang-Hee BaeSang Chul Lee

Publisher 

Elsevier

Issue Date 

2009

Citation 

Biochemical and Biophysical Research Communications, vol. 383, no. 2, pp. 252-257

Keywords 

adipocyteadipogenesisdifferentiationhuman mesenchymal stem cellsprotein tyrosine phosphatase RQ

Abstract 

Mesenchymal stem cells (MSCs) are self-renewable multipotent progenitor cells with the capacity to differentiate into several distinct mesenchymal lineages. While MSCs display significant potential in tissue engineering and therapeutic applications, the regulatory mechanisms underlying the differentiation of these cells are yet to be established. Phosphorylation is a post-translational modification that plays a significant role in diverse biological phenomena. In this study, to mine the protein tyrosine phosphatases (PTPs) involved in adipogenesis of human MSCs, differential expression of human PTPs was examined using RT-PCR analysis. Among the 107 human PTPs, PTP-RQ was dramatically downregulated during the early phase of adipogenesis. PTP-RQ is classified as a receptor-type III PTP with phosphatidylinositol phosphatase (PIPase) activity. Overexpression of PTP-RQ consistently led to reduced differentiation of MSCs into adipocytes via decreasing the phosphatidyl inositol phosphate level in cells, and consequently downregulating Akt/PKB phosphorylation. Our results collectively suggest that PTP-RQ is a useful target protein for regulating the differentiation of MSCs into adipocytes, and may be used to develop novel drugs for the treatment of obesity.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2009.04.001

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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