상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Purification and characterization of recombinant human erythropoietin from milk of transgenic pigs

Authors 

Eun Gyo LeeSeung-hui LeeKyung Mi ParkJ E BaekSeon Hee YeonJ K ParkW K ChangJoon Ki JungBong Hyun Chung

Issue Date 

2009

Citation 

Journal of Chemical Technology Biotechnology, vol. 84, no. 5, pp. 643-649

Keywords 

transgenic pigrecombinant human erythropoietin(rhEPO)purificationglycosylation

Abstract 

BACKGROUND: Human erythropoietin (hEPO), a hydrophobic acidic glycoprotein responsible for the regulation of red blood cell production in mammals, is used for the treatment of anemia. In general, the purification of transgenic animal-derived therapeutic proteins is not easy due to their low titer concentrations and abundant contaminant proteins. For the first time, here the purification and characterization of rhEPO from themilk of transgenic pigs are described. RESULTS: The rhEPO was purified by heparin chromatography, reverse-phase chromatography, and gel filtration chromatography, resulting in a 16.5% yield and >98% purity. The rhEPO purified from the milk of transgenic pigs contained less acidic isoforms andwas underglycosylated in contrast to CHO-derived rhEPO. Cell proliferation of the F-36/EPO-dependent cell line was proportional to the dose of transgenic pig-derived rhEPO. CONCLUSION:Transgenicpig-derivedrhEPOwithhighpuritywas achieved after three-step chromatographyfollowing two-step precipitation. The transgenic pig-derived rhEPO was demonstrated to have comparable potency with CHO-derived rhEPO. Transgenic pig-derived rhEPO may not be therapeutically feasible because of different glycosylation, and thus further studies are required to elucidate the effect of this aberrant glycosylation on the biological activity and stability in vivo.

ISSN 

0268-2575

Link 

http://dx.doi.org/10.1002/jctb.2094

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)