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Title 

Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction

 

돼지 ADAM3 disintegrin domain이 정자와 난자의 결합에 중요

Authors 

Ekyune KimK E ParkJi Su KimDong Chul BaekJ W LeeSang Rae LeeMyeong Su KimSang-Hyun KimC S KimD B KooH S KangZ Y RyooKyu Tae Chang

Publisher 

Society for Reproduction and Development

Issue Date 

2009

Citation 

Journal of Reproduction Development, vol. 55, no. 2, pp. 156-162

Abstract 

In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.

ISSN 

0916-8818

Link 

http://dx.doi.org/10.1262/jrd.20134

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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