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Title 

An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli

 

대장균에서 외래유전자를 항시 고발현 시키기 위한 발현 벡터

Authors 

Jeong Woo SeoWon-Kyung HongD RairakhwadaPil-Soo SeoMin-Ho ChoiK B SongSang Ki RheeChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2009

Citation 

Biotechnology Letters, vol. 31, no. 6, pp. 877-881

Keywords 

escherichia coligene expressionlevansucrase genepromoterrahnella aquatilis

Abstract 

The levansucrase gene (lsrA) from Rahnella aquatilis was strongly expressed in a constitutive manner in Escherichia coli when cloned into a pBluescript KS-based pRL1CP plasmid vector. The native promoter upstream of lsrA and the lacZ promoter cooperatively enhanced the expression of lsrA to a level that was comparable to that of the T7 promoter, which is used in commercial pET expression vector system. A putative rho-independent transcription termination signal downstream of lsrA was crucial for gene expression. This plasmid vector also proved to be applicable for efficient expression of other foreign genes in E. coli.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-009-9941-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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