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Title 

Analysis of β-(1→3)(1→6)-glucan produced by Aureobasidium pullulans IMS-822

 

Aureobasidium pullulans IMS-822가 생산하는 β-(1→3)(1→6)-glucan의 특성 분석

Authors 

Seog June LeeK H AhnChan Sun ParkByung Dae YoonMin-Soo Kim

Publisher 

The Microbiological Society of Korea

Issue Date 

2009

Citation 

Korean Journal of Microbiology, vol. 45, no. 1, pp. 63-68

Keywords 

β-(1→3)(1→6)-glucanaqueous conformationaureobasidium sp.viscosity-average molecular weight

Abstract 

The exo-polysaccharide producing microorganism, Aureobasidium pullulans IMS-822, was isolated and identified from soil. The viscosity-average molecular weight of exo-polysaccharide was calculated as 8.9×105 by Mark-Houwink equation. The sugar component of exo-polysaccharide was determined as glucose by HPLC analysis. The IR spectra indicated that the exo-polysaccharide has an absorption peak at 890 cm-1 for the β-configuration of D-glucan. The 13C NMR signal at δ 86.62 ppm arose from the substituted C-3 of glucose. The signal at δ 72.11 ppm was assigned to C-6 of branched β-(1→3)-D-glucosyl residues. Viscosity and Congo red reaction indicated that β-(1→3)(1→6)-glucan produced by A. pullulans IMS-822 has a highly ordered hydrogen-bond dependent conformation in aqueous solution, which collapses in strong alkaline solution.

ISSN 

0440-2413

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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