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Title 

Spore display using Bacillus thuringiensis exosporium protein InhA

Authors 

T J ParkSoo Keun ChoiHeung Chae JungS Y LeeJae Gu Pan

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2009

Citation 

Journal of Microbiology and Biotechnology, vol. 19, no. 5, pp. 495-501

Abstract 

A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and beta-galactosidase as model proteins were fused to the Cterminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.0802.163

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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