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Title | High-level production of lycopene in metabolically engineered E. coli |
Authors | S W Kim; J B Kim; J M Ryu; Joon Ki Jung; J H Kim |
Publisher | Elsevier |
Issue Date | 2009 |
Citation | Process Biochemistry, vol. 44, no. 8, pp. 899-905 |
Keywords | inocula dependence; lycopene; pantoea ananatis; plasmid copy number; synthetic operon; temperature-shift |
Abstract | Recombinant lycopene was generated by utilizing metabolically engineered Escherichia coli with yields being dependent upon inocula state. Yields were especially low in the case of cultures harboring high-copy plasmids that were established with inocula at the stationary growth phase. On the other hand, cultures derived using low-copy plasmid, however, yielded high amounts of lycopene irrespective of inocula state. Nevertheless, it showed still an inocula dependence pattern in lycopene productivity (mg/l/h). To further increase lycopene productivity, we applied a temperature-shift culture technique (37 → 25 °C). Using this method, we effectively enhanced lycopene productivity without any problematic phenomena. As a result, we were able to increase lycopene yield by approximately 20% compared to previous culture methods. In the present study, we were able to reach a final lycopene yield up to 260 mg/l for 60 h, which corresponds to the highest titer to date for the production of lycopene in E. coli. |
ISSN | 0032-9592 |
Link | |
Appears in Collections | |
Registered Date |
2017-04-19 |
