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Title 

High-level production of lycopene in metabolically engineered E. coli

Authors 

S W KimJ B KimJ M RyuJoon Ki JungJ H Kim

Publisher 

Elsevier

Issue Date 

2009

Citation 

Process Biochemistry, vol. 44, no. 8, pp. 899-905

Keywords 

inocula dependencelycopenepantoea ananatisplasmid copy numbersynthetic operontemperature-shift

Abstract 

Recombinant lycopene was generated by utilizing metabolically engineered Escherichia coli with yields being dependent upon inocula state. Yields were especially low in the case of cultures harboring high-copy plasmids that were established with inocula at the stationary growth phase. On the other hand, cultures derived using low-copy plasmid, however, yielded high amounts of lycopene irrespective of inocula state. Nevertheless, it showed still an inocula dependence pattern in lycopene productivity (mg/l/h). To further increase lycopene productivity, we applied a temperature-shift culture technique (37 → 25 °C). Using this method, we effectively enhanced lycopene productivity without any problematic phenomena. As a result, we were able to increase lycopene yield by approximately 20% compared to previous culture methods. In the present study, we were able to reach a final lycopene yield up to 260 mg/l for 60 h, which corresponds to the highest titer to date for the production of lycopene in E. coli.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/j.procbio.2009.04.018

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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