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Title 

Over-expression of Reticulon 3 (RTN3) enhances TRAIL-mediated apoptosis via up-regulation of death receptor 5 (DR5) and down-regulation of c-FLIP

Authors 

J T LeeT J LeeC H KimNam-Soon KimT K Kwon

Publisher 

Elsevier

Issue Date 

2009

Citation 

Cancer Letters, vol. 279, no. 2, pp. 185-192

Keywords 

RTN3TRAILapoptosisDR5c-FLIP

Abstract 

Reticulons (RTNs) are a group of integral membrane proteins that have no homology to other known apoptosis-related domains. Herein, we found that RTN3 overexpressing Caki cells were sensitive to TRAIL-mediated apoptosis. RTN3-induced down-regulation of c-FLIP was recovered by pan-caspase inhibitor, z-VAD to basal levels in TRAIL-treated cells. The forced expression of c-FLIP attenuated the TRAIL-mediated apoptosis in RTN3 overexpressing cells. In addition, RTN3 over-expression provoked the enhanced protein levels in DR4 and DR5 as well as levels in DR5 surface protein but slight increase in DR4 surface protein. RTN3-mediated enhancement of TRAIL-induced apoptosis was markedly blocked by the DR5/Fc chimera or DR5 siRNA, indicating that the sensitization by RTN3 was mainly mediated through interactions of TRAIL with its receptors, DR5. Over-expression of RTN3 also enhanced TNF-a and Fas-mediated apoptosis. Taken together, over-expression of RTN3 might increase DR5 surface protein and concomitantly more activate caspase pathways, which cause the c-FLIP cleavage and enhancement of TRAIL-mediated apoptosis.

ISSN 

0304-3835

Link 

http://dx.doi.org/10.1016/j.canlet.2009.01.035

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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