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Title 

Protein tyrosine phosphatase 1B inhibitory by dammaranes from Vietnamese Giao-Co-Lam tea

Authors 

T M HungD M HoangJ C KimH S JangJong Seog AhnB S Min

Publisher 

Elsevier

Issue Date 

2009

Citation 

Journal of Ethnopharmacology, vol. 124, no. 2, pp. 240-245

Keywords 

gynostemma pentaphyllumcucurbitaceaedammaranetriterpeneprotein tyrosine phosphatase 1B activity

Abstract 

Ethnopharmacological relevance: Gynostemma pentaphyllum (Thunb.) tea was used in Vietnamese folk medicine as anti-diabetic agent. Aim of the study: This studywas aimed to investigate the inhibitory activities of fractions and constituents isolated from Gynostemma pentaphyllum on protein tyrosine phosphatase 1B (PTP1B) since it has been proposed as a treatment therapy for type 2 diabetes and obesity. Materials and methods: The 70% EtOH extract, CHCl3 fraction, EtOAc fraction, BuOH fraction, and seven isolated dammarane triterpeneswere evaluated for their inhibitory activity in protein phosphatase enzymes (PTP1B and VHR). Results: CHCl3-soluble fraction showed a dose-dependent inhibitory activity of the PTP1B enzyme with the IC50 value of 30.5μg/mL. Among seven tested compounds, compounds 6 showed the most potent PTP1B inhibitory activity with IC50 value of 5.3±0.4μM compared to a range 15.7.28.5μM for the other six compounds. The inhibition mode of 6 was competitive toward p-NPP with a Ki value of 2.8μM. Conclusion: These study results suggested that the PTP1B inhibitory activity of these dammaranes may enable this plant to play an important role in the treatment of diabetes.

ISSN 

0378-8741

Link 

http://dx.doi.org/10.1016/j.jep.2009.04.027

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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