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Title 

Isolation and characterization of a cellulase-free endo-beta-1,4-xylanase produced by an invertebrate-symbiotic bacterium, Cellulosimicrobium sp. HY-13

Authors 

Do Young KimMi Kyoung HanJong Suk LeeHyun Woo OhDoo Sang ParkD H ShinKyung Sook BaeKwang Hee SonHo Yong Park

Publisher 

Elsevier

Issue Date 

2009

Citation 

Process Biochemistry, vol. 44, no. 9, pp. 1055-1059

Keywords 

cellulosimicrobium sp. HY-13earthwormeisenia fetidaendo-b-1,4-xylanaseinvertebratexylooligosaccharides

Abstract 

A xylanolytic bacterium, Cellulosimicrobium sp. HY-13, was isolated from the digestive tract of an earthworm, Eisenia fetida. The purified cellulase-free endo-b-1,4-xylanase (XylK) produced by strain HY-13 was found to contain an N-terminal amino acid sequence of APSTLEAAAE and to have a relative molecular mass of 36 kDa. It was most active at pH 6.0 and 55 8C and had Vmax and Km values toward oat spelt xylan of 4067 IU/mg and 2.78 mg/ml, respectively. XylK primarily degraded xylan to a series of xylooligosaccharides composed of xylobiose to xylotetraose, but it could not further hydrolyze xylobiose to xylose. The results of the present study suggest that the relatively highly active XylK lacking exoxylanolytic activity is a promising candidate for the efficient production of non-digestible xylooligosaccharides that may have beneficial effects to gastrointestinal health via promotion of the growth of probiotics.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/j.procbio.2009.05.005

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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