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Title 

Upregulation of the cysteine protease inhibitor, cystatin SN, contributes to cell proliferation and cathepsin inhibition in gastric cancer

Authors 

Eun Hwa ChoiJong Tae KimJ H KimS Y KimEun Young SongJae Wha KimSeon-Young KimYoung Il YeomI H KimHee Gu Lee

Publisher 

Elsevier

Issue Date 

2009

Citation 

Clinica Chimica Acta, vol. 406, no. 1, pp. 45-51

Keywords 

CST1cystatin SNcathepsinproliferationgastric cancer

Abstract 

Background: Cysteine proteases like cathepsins are widely distributed proteolytic enzymes and form tight equimolar complexes with cystatins at their active sites. Among cystatins, CST1, encoding cystatin SN, is a member of the type 2 salivary cystatin family found in a variety of fluids and secretions, including plasma, tears, and saliva. CST1 was identified as an upregulated gene in gastric cancer tissues compared to noncancerous regions using our Affymetrix GeneChip microarray. Methods: The upregulation of CST1 in gastric cancer was analyzed using RT-PCR (n=15), immnohistochemistry, and clinicopathological (n=77) analysis. CST1-siRNA was used for the suppression of CST1 gene expression and cathepsin proteolytic activity was assayed. Results: CST1 was upregulated in cancerous lesions of gastric cancer tissues compared to noncancerous regions and clinicopathological analysis showed a significant correlation between high expression of CST1 and pTNM stage (p=0.044). In CST1-siRNA transfected cells, cell proliferation was reduced and the proteolytic activity of cathepsins was increased. Conclusions: CST1 might be highly involved in gastric tumorigenesis and regulate the proteolytic activity of cysteine proteases.

ISSN 

0009-8981

Link 

http://dx.doi.org/10.1016/j.cca.2009.05.008

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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