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Title 

Bis is involved in glial differentiation of P19 cells induced by retinoic acid

Authors 

J S YoonM Y LeeJ S LeeChan Sun ParkH J YounJ H Lee

Publisher 

The Korean Physiological Society

Issue Date 

2009

Citation 

Korean Journal of Physiology & Pharmacology, vol. 13, no. 3, pp. 251-256

Keywords 

BisGFAPGliogenesisNeuronal differentiationP19

Abstract 

Previous observations suggest that Bis, a Bcl-2-binding protein, may play a role the neuronal and glial differentiation in vivo. To examine this further, we investigated Bis expression during the in vitro differentiation of P19 embryonic carcinoma cells induced by retinoic acid (RA). Western blotting and RT-PCR assays showed that Bis expression was temporarily decreased during the free floating stage and then began to increase on day 6 after the induction of differentiation. Double immunostaining indicated that Bis-expressing cells do not express several markers of differentiation, including NeuN, MAP-2 and Tuj-1. However, some of the Bis-expressing cells also were stained with GFAP-antibodies, indicating that Bis is involved glial differentiation. Using an shRNA strategy, we developed bis-knock down P19 cells and compared them with control P19 cells for the expression of NeuroD, Mash-1 and GFAP during RA-induced differentiation. Among these, only GFAP induction was significantly attenuated in P19-dnbis cells and the population showing GFAP immunoreactivity was also decreased. It is noteworthy that distribution of mature neurons and migrating neurons was disorganized, and the close association of migrating neuroblasts with astrocytes was not observed in P19-dnbis cells. These results suggest that Bis is involved in the migration-inducing activity of glial cells.

ISSN 

1226-4512

Link 

http://dx.doi.org/10.4196/kjpp.2009.13.3.251

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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