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Title 

Inhibition of GSK-3beta enhances reovirus-induced apoptosis in colon cancer cells

Authors 

H J MinSang Seok KohI R ChoR SrisutteeE H ParkB H JhunY G KimS OhJ E KwakR N JohnstonY H Chung

Publisher 

Spandidos Publications

Issue Date 

2009

Citation 

International Journal of Oncology, vol. 35, no. 3, pp. 617-624

Keywords 

reovirusglycogen synthase kinase-3ßß-cateninnuclear factor-κBcolon cancer

Abstract 

Reovirus functions as an oncolytic agent for many types of cancer including colon cancer. Although most studies have emphasized the role of activated Ras signaling in enhancing reoviral oncolysis in susceptible cells, we note that many colon cancers also display elevated ß-catenin. Thus, it is possible that enhanced ß-catenin may augment reoviral susceptibility in colon cancer cells. To explore this hypothesis, HEK293 cells were treated with the glycogen synthase kinase (GSK)-3ß inhibitor LiCl, thereby inducing ß-catenin, followed by reoviral infection. Co-administration with LiCl indeed enhanced cell death compared to reovirus infection alone, but this was not associated with elevated reoviral replication. Similarly, HEK293 cells expressing the Frizzled-1 receptor in Wnt3a-conditioned medium also showed reovirus replication equivalent to that in cells in control medium, further suggesting that up-regulation of ß-catenin does not enhance the replication of reovirus. Instead, we observed that inhibition of GSK-3ß with LiCl decreased reovirus-induced NF-κB activation, leading to accelerated apoptosis via

ISSN 

1019-6439

Link 

http://dx.doi.org/10.3892/ijo_00000373

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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