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Title 

Species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the prfA virulence gene cluster

Authors 

H J JungS H ParkS D HaK H LeeD H ChungC H KimMin-Gon KimK Y KimK S Kim

Publisher 

Japan Society for Bioscience, Biotechnology and Agrochemistry

Issue Date 

2009

Citation 

Bioscience Biotechnology and Biochemistry, vol. 73, no. 6, pp. 1412-1415

Keywords 

Listeria monocytogenesPCRprfA virulence gene cluster

Abstract 

Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction(PCR) assays to detect and specifically identify L monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.

ISSN 

0916-8451

Link 

http://dx.doi.org/10.1271/bbb.70252

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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