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Title 

A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose

 

아가로스를 이용한 새로운 결정화 방법

Authors 

Tae Woong WhonY H LeeDong-Shan AnH K SongSong-Gun Kim

Publisher 

International Union of Crystallography

Issue Date 

2009

Citation 

Journal of Applied Crystallography, vol. 42, no. 5, pp. 975-976

Keywords 

Agarose gelCrystallizationGlucose isomeraseLysozymeProtein crystallographySolidified reservoirsThaumatinUpside-down sitting-drop vapor diffusion

Abstract 

A simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the upside-down sitting-drop method could produce single crystals with all the benefits of the hanging-drop crystallization method.

ISSN 

0021-8898

Link 

http://dx.doi.org/10.1107/S0021889809028805

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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