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Title 

Cascade enzyme-linked immunosorbent assay (CELISA)

Authors 

Young-Mi LeeY JeongH J KangSang Jeon ChungBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2009

Citation 

Biosensors & Bioelectronics, vol. 25, no. 2, pp. 332-337

Keywords 

BiosensorsCELISAELISAEnzyme cascadeImmunoassays

Abstract 

Immunoassays are representative biochemical detection methods. Among them, sandwich-type immunoassays, typified by sandwich ELISA, have used in disease diagnosis or biochemical detection with high target selectivity. Horseradish peroxidase and alkaline phosphatase have been typically used for signal amplification in ELISA. Recently developed sandwich-type immunoassays such as biobarcode immunoassays, immuno-PCR, and immuno-RCA have improved sensitivity by changing mainly the signal amplification method. To develop a novel amplification method in ELISA, an enzyme-cascading system was incorporated into an ELISA, and the new assay is termed a cascading enzyme-linked immunosorbent assay (CELISA). This CELISA includes a trypsinogen-enterokinase combination as the cascading enzyme system, and was used to detect alpha-fetoprotein (AFP), which is a liver cancer marker, and prostate-specific antigen (PSA). Using a colorimetric reagent for signal generation, CELISA had 0.1-10 pM limits-of-detection for AFP and PSA in whole human serum and assay buffers, depending on the platform, well plate, or microbead type used. This study represents the first example that incorporated an enzyme cascading step in an ELISA system, resulting in successful signal amplification with sensitive detection of pathogenic antigens in serum.

ISSN 

0956-5663

Link 

http://dx.doi.org/10.1016/j.bios.2009.07.010

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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