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Title 

Efficient, galactose-free production of Candida antarctica lipase B by GAL10 promoter in Δgal80 mutant of Saccharomyces cerevisiae

Authors 

Jea Ik WhangJungoh AhnC S ChunY J SonHong-Weon LeeEui Sung Choi

Publisher 

Elsevier

Issue Date 

2009

Citation 

Process Biochemistry, vol. 44, no. 10, pp. 1190-1192

Keywords 

Δgal80Candida antarctica lipase B (CalB)GAL10 promoterGalactose-freeRecombinant proteinSaccharomyces cerevisiae

Abstract 

An efficient yeast gene expression system with GAL10 promoter that does not require galactose as an inducer was developed using Δgal80 mutant strain of Saccharomyces cerevisiae. We constructed several combinations of gal mutations (Δgal1, Δgal80, Δmig1, Δmig2, and Δgal6) of S. cerevisiae and tested for their effect on efficiency of recombinant protein production by GAL10 promoter using a lipase, Candida antarctica lipase B (CalB), as a reporter. While the use of Δgal1 mutant strain required the addition of a certain amount of galactose to the medium, Δgal80 mutant strain did not require galactose. Furthermore, it was found that the recombinant CalB could be produced more efficiently (1.6-fold at 5 L-scale fermentation) in Δgal80 mutant strain than in the Δgal1 mutant. The Δgal80 mutant strain showed glucose repressible mode of expression of GAL10 promoter. Using Δgal80 mutant strain of S. cerevisiae, CalB was efficiently produced in a glucose-only fermentation at volumes up to 500 L.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/j.procbio.2009.06.009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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