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Title 

Isolation and characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. BR-2

 

슈와넬라속 해양미생물 유래 EPA 생합성 유전자군의 분리와 특성 규명

Authors 

P S SeoChul Ho KimOh Suk KwonB K HurJeong Woo Seo

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2009

Citation 

Journal of Microbiology and Biotechnology, vol. 19, no. 9, pp. 881-887

Keywords 

Biosynthesis gene clusterEicosapentaenoic acidPKS-like moduleShewanella sp. BR-2

Abstract 

Forty-four eicosapentaenoic acid (EPA)-producing microbial strains were isolated from the intestines of marine fishes. Among them, one strain showing a maximum level of EPA (4.78% of total fatty acids) was identified as Shewanella sp. BR-2 on the basis of its 16S rRNA sequence. The EPA content reached a maximum level during the mid-exponential phase of cell growth, and gradually decreased with further growth of the cells. A cosmid DNA including the EPA biosynthesis gene cluster consisting of pfaA-E was isolated from a cosmid library of genomic DNA of Shewanella sp. BR-2, named pCosEPA-BR2. An E. coli clone harboring pCosEPA-BR2 produced EPA at a maximum level of 7.5% of total fatty acids, confirming the EPA biosynthesis activity of the cloned gene cluster.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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