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Title 

Comparison of several methods for effective lipid extraction from microalgae

Authors 

Jae Yon LeeChan YooSo Young JunChi-Yong AhnHee-Mock Oh

Publisher 

Elsevier

Issue Date 

2010

Citation 

Bioresource Technology, vol. 101, no. 1, pp. s75-s77

Keywords 

BiodieselCell disruptionLipid extractionMicroalgae

Abstract 

Various methods, including autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, were tested to identify the most effective cell disruption method. The total lipids from Botryococcus sp., Chlorella vulgaris, and Scenedesmus sp. were extracted using a mixture of chloroform and methanol (1:1). The lipid contents from the three species were 5.4-11.9, 7.9-8.1, 10.0-28.6, 6.1-8.8, and 6.8-10.9 g L-1 when using autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, respectively. Botryococcus sp. showed the highest oleic acid productivity at 5.7 mg L-1 d-1 when the cells were disrupted using the microwave oven method. Thus, among the tested methods, the microwave oven method was identified as the most simple, easy, and effective for lipid extraction from microalgae.

ISSN 

0960-8524

Link 

http://dx.doi.org/10.1016/j.biortech.2009.03.058

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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