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Title 

Cloning, expression, and characterization of a new deoxyribose 5-phosphate aldolase from Yersinia sp. EA015

 

Yersinia로 부터 신규 알도라제의 클로닝 발현 및 특징화

Authors 

Y M KimYoung Hyo ChangNack-Shick ChoiY KimJae Jun SongJoong Su Kim

Publisher 

Elsevier

Issue Date 

2009

Citation 

Protein Expression & Purification, vol. 68, no. 2, pp. 196-200

Keywords 

AcetaldehydeAldolaseDERAYersinia sp.

Abstract 

A new deoC gene encoding deoxyribose 5-phosphate aldolase (DERA) was identified in Yersinia sp. EA015 isolated from soil. The DERA gene had an open reading frame (ORF) of 672 base pairs encoding 223 amino acids to yield a protein of molecular mass 24.8 kDa. The amino acid sequence was 94% identical to that of DERA from Yersinia intermedia ATCC 29909. DERA was over-expressed in Escherichia coli and purified using Ni-NTA affinity chromatography. The specific activity was 137 μmol/min/mg. The Michaelis constant (km value) of DERA was 9.1 mM. DERA was optimally active at pH 6.0 and 50 °C. DERA was tolerant to a high concentration (300 mM) of acetaldehyde.

ISSN 

1046-5928

Link 

http://dx.doi.org/10.1016/j.pep.2009.06.002

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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