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Title 

Large-scale expression in Escherichia coli and efficient purification of precursor and active caspase-7 by introduction of thrombin cleavage sites

Authors 

Young-Mi LeeHyo Jin KangM JangMoonil KimKwang-Hee BaeSang Jeon Chung

Publisher 

Elsevier

Issue Date 

2010

Citation 

Protein Expression & Purification, vol. 69, no. 1, pp. 29-33

Abstract 

Caspases are a family of cysteine proteases that have critical roles in the apoptotic pathway. Caspase-7 is a well-known apoptotic effector that cleaves a variety of cellular substrates, and is known to be an important target in the treatment of many diseases. For efficient research, large amounts of the protein are required. However, it has been difficult to obtain sufficient quantities of either the precursor or active caspase-7 from Escherichia coli strain. In the present study, we constructed thrombin-activatable caspase-7 precursors by changing the auto-activation sites of the caspase-7 precursor into sequences susceptible to thrombin cleavage. These engineered precursors were highly expressed as soluble proteins in E. coli, and were easily purified by affinity chromatography (to levels of 10-15 mg per liter of E. coli culture), and were then readily activated by treatment with thrombin. In vitro cleavage assays and kinetic analyses revealed that the engineered active caspase-7 proteins had characteristics similar to those of wild-type caspase-7. This novel method is valuable for obtaining both precursor and active caspase-7, thereby contributing to the development of caspase-7-specific drugs to treat various diseases, including cancer and neurodegenerative conditions.

ISSN 

1046-5928

Link 

http://dx.doi.org/10.1016/j.pep.2009.09.014

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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