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Title 

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01

Authors 

Do Young KimDoo Sang ParkS B KwonM G ChungKyung Sook BaeHo Yong ParkY H Rhee

Publisher 

Microbiological Society of Korea

Issue Date 

2009

Citation 

Journal of Microbiology, vol. 47, no. 5, pp. 651-656

Keywords 

BurkholderiaInsect-symbiotic microorganismLevulinic acidPHA synthasePoly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)]Polyhydroxyalkanoates (PHAs)

Abstract 

Burkholderia sp. IS-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)] copolyesters with a high molar fraction of 3HV was isolated from the gut of the adult longicorn beetle, Moechotypa diphysis. The strain IS-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3HB-co-86.5 mol% 3HV) copolyester when cultivated on a mixture of gluconate (20 g/L) and levulinic acid (12.5 g/L). In this case, the content of the copolyester in the cells was approximately 60.0%. The compositions of the copolyesters were easily regulated by altering the molar ratio of gluconate and levulinic acid in the medium. The organism was found to possess a class I PHA synthase (PhaC) gene (1,881 bp) that encodes a protein with a deduced molecular mass of 68,538 Da that consists of 626 amino acids. The PhaC of this organism was most similar to that of B. cenocepacia PC184 (92% similarity).

ISSN 

1225-8873

Link 

http://dx.doi.org/10.1007/s12275-009-0109-7

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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