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Title 

Beta-glucan enhanced apoptosis in human colon cancer cells SNU-C4

 

베타글루칸이 인간결장암 암세포 SNU-C4 에서 세포괴사를 증진

Authors 

M J KimS Y HongS K KimC CheongH J ParkH K ChunK H JangByung Dae YoonChul Ho KimS A Kang

Publisher 

The Korean Nutrition Society

Issue Date 

2009

Citation 

Nutrition Research and Practice, vol. 3, no. 3, pp. 180-184

Abstract 

The apoptotic effect of bacteria-derived beta-glucan was investigated in human colon cancer cells SNU-C4 using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. beta-Glucan of 10, 50, and 100 microg/mL decreased cell viability in a dose-dependent manner with typical apoptotic characteristics, such as morphological changes of chromatin condensation and apoptotic body formation from TUNEL assay. In addition, beta-glucan (100 microg/mL) decreased the expression of Bcl-2 by 0.6 times, whereas the expression of Bax and Caspase-3 were increased by 3.1 and 2.3 times, respectively, compared to untreated control group. Furthermore, the caspase-3 activity in the beta-glucan-treated group was significantly increased compared to those in control group (P < 0.05). Bacterial derived beta-glucan could be used as an effective compound inducing apoptosis in human colon cancer.

ISSN 

1976-1457

Link 

http://dx.doi.org/10.4162/nrp.2009.3.3.180

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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