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Title 

Stable plastid transformation in Nicotiana benthamiana

Authors 

S J DavarpanahS H JungY J KimY I ParkSung Ran MinJang Ryol LiuWon Joong Chung

Publisher 

The Botanical Society of Korea

Issue Date 

2009

Citation 

Journal of Plant Biology, vol. 52, no. 3, pp. 244-250

Keywords 

Nicotiana benthamianaPlastid transformation

Abstract 

Plastids from Nicotiana benthamiana were transformed with the vector for dicistronic expression of two genes-aminoglycoside 3'-adenyltransferase (aadA) and green fluorescent protein (gfp)-in the plastids of Nicotiana tabacum. Transplastomic shoots exhibited green fluorescence under UV light. Transformation efficiencies were similar between species. Although the border sequence (trnI and trnA) for homologous recombination to transform the plastid genome of N. benthamiana was identical to that sequence of N. tabacum, the exception was a 9-bp addition in the intron of trnI. This indicated that the N. tabacum sequence used as a border region for recombination was sufficient to insert the foreign gene into the target site between the trnI and trnA of N. benthamiana with similar efficiency. Southern blot analysis detected the presence of aadA and gfp between trnI and trnA in the plastid genome of N. benthamiana. Northern and western blot analyses revealed high expression of gfp in the plastids from petals and leaves. Our results suggest that the plastid transformation system established here is applicable to investigations of the interactions between plastid and nucleus in N. benthamiana.

ISSN 

1226-9239

Link 

http://dx.doi.org/10.1007/s12374-009-9023-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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