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Title 

Formation of vitamin A lipid droplets in pancreatic stellate cells requires albumin

Authors 

N KimW YooJ LeeH KimH LeeY S KimDong Uk KimJ Oh

Publisher 

BMJ Publishing Group

Issue Date 

2009

Citation 

Gut, vol. 58, no. 10, pp. 1382-1390

Abstract 

Objective: Quiescent pancreatic stellate cells (PSCs) store vitamin A as cytoplasmic lipid droplets, and, when activated by profibrogenic stimuli, they transform into myofibroblast-like cells characterised by the loss of vitamin A droplets. Activation of stellate cells is central to fibrogenesis, but the mechanism for the formation of vitamin A droplets and its relationship to stellate cell activation remain unclear. Methods: With use of cultured PSCs, an attempt was made to characterise the function of albumin endogenously expressed in stellate cells. Results: Albumin is endogenously expressed in quiescent PSCs, localised in cytoplasmic lipid droplets, and its levels are markedly reduced after stellate cell activation. Continuous albumin expression in stellate cells is sufficient to maintain their fat-storing phenotype even after cell passages and renders cells resistant to the activating effects of transforming growth factor β (TGFβ). Forced expression of albumin in PSCs after passage 2 (activated PSCs) induced the re-appearance of lipid droplets and phenotypic changes, which were previously reported with retinol treatment. Retinol increases albumin synthesis in activated PSCs and the suppression of albumin expression using small interfering RNA (siRNA) abolishes retinol-induced effects. Conclusions: The data demonstrate a novel role for albumin in the formation of cytoplasmic vitamin A lipid droplets in stellate cells, and suggest that albumin may have a direct influence on stellate cell activation

ISSN 

0017-5749

Link 

http://dx.doi.org/10.1136/gut.2008.170233

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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