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Title 

Catalytic properties of a GH10 endo-β-1,4-xylanase from Streptomyces thermocarboxydus HY-15 isolated from the gut of Eisenia fetida

Authors 

Do Young KimMi Kyung HanHyun Woo OhDoo Sang ParkSu Jin KimSeung Goo LeeD H ShinKwang Hee SonKyung Sook BaeHo Yong Park

Publisher 

Elsevier

Issue Date 

2010

Citation 

Journal of Molecular Catalysis B:Enzymatic, vol. 62, no. 1, pp. 32-39

Keywords 

Endo-β-1,4-xylanaseGlycoside hydrolase family 10p-NitrophenylcellobiosidePNP-cellobiosideStreptomyces thermocarboxydus HY-15

Abstract 

A novel GH10 endo-β-1,4-xylanase (XylG) gene from Streptomyces thermocarboxydus HY-15, which was isolated from the gut of Eisenia fetida, was cloned, over-expressed, and characterized. The XylG gene (1182 bp) encoded a polypeptide of 393 amino acids with a deduced molecular mass of 43,962 Da and a calculated pI of 6.74. The primary structure of XylG was 69% similar to that of Thermobifida fusca YX endo-β-1,4-xylanase. It was most active at pH 6.0 and 55 °C. The susceptibilities of xylans to XylG were as follows: oat spelt xylan > birchwood xylan > beechwood xylan. The XylG also showed high activity (474 IU/mg) toward p-nitrophenylcellobioside. Moreover, at pH 6.0 and 50 °C, the Vmax and Km values of the XylG were 127 IU/mg and 2.51 mg/ml, respectively, for oat spelt xylan and 782 IU/mg and 5.26 mM, respectively, for p-nitrophenylcellobioside. A homology model indicated that XylG folded to form a (β/α)8-barrel with two catalytic residues of an acid/base (Glu181) and a nucleophile (Glu289). The formation of a disulfide bond between Cys321 and Cys327 were predicted by homology modeling.

ISSN 

1381-1177

Link 

http://dx.doi.org/10.1016/j.molcatb.2009.08.015

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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