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Title 

Processing and subcellular localization of ADAM2 in the Macaca fascicularis testis and sperm

Authors 

Ekyune KimJae Woong LeeD C BaekSang Rae LeeMyeong Su KimSang-Hyun KimC S KimZ Y RyooH S KangKyu Tae Chang

Publisher 

Elsevier

Issue Date 

2010

Citation 

Animal Reproduction Science, vol. 117, no. 1, pp. 155-159

Keywords 

FertilinFertilizationMacaca fascicularis ADAM2Spermatogenesis

Abstract 

Fertilin, a heterodimeric protein complex composed of ADAM1 and ADAM2 located on the sperm surface, is involved in sperm-egg interaction. In our study, we examined the physiological processing and subcellular localization of M. fascicularis ADAM2 during spermatogenesis in the testis and epididymal tract. M. fascicularis ADAM2 was initially synthesized as a 100 kDa precursor in testicular germ cells. After passing into 50 kDa intermediate form in the epididymal tracts, the precursor form was finally processed into a 47 kDa protein in sperm. We found that M. fascicularis ADAM2 is localized on the sperm surface and contributes to the formation of a candidate fertilin complex. In particular, Far-Western blot analysis revealed that M. fascicularis ADAM2 cystein-rich domain may be related to protein-protein interaction. Therefore, the cystein-rich domain of ADAM2 could provide a mechanism to form a fertilin complex.

ISSN 

0378-4320

Link 

http://dx.doi.org/10.1016/j.anireprosci.2009.04.002

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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