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Title 

Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation

 

세포막지방산 조성의 제어를 통한 에탄올 내성 증진

Authors 

L H LuoPil-Soo SeoJeong Woo SeoSun Yeon HeoD H KimChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2009

Citation 

Biotechnology Letters, vol. 31, no. 12, pp. 1867-1871

Keywords 

DesaturaseEscherichia coliEthanol toleranceFabAFatty acids composition

Abstract 

To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding β-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-009-0092-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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