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Title 

Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose

Authors 

Suk Weon KimMyung Jin OhJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2009

Citation 

Plant Biotechnology Reports, vol. 3, no. 3, pp. 199-203

Keywords 

Rosa rugosaSomatic embryogenesisTissue culture

Abstract 

Rugosa rose (Rosa rugosa) is cultivated as a garden flower and an important genetic resource for the breeding of roses (R. hybrida). This study describes culture conditions for high frequency plant regeneration from zygotic embryo explants via somatic embryogenesis in rugosa rose. Mature zygotic embryo, cotyledon, and radicle explants formed embryogenic calluses at frequencies of 38, 6.7, and 8.8% when cultured on half-strength Murashige and Skoog medium (1/2MS) supplemented with 2.26, 9.05, and 9.05 μM 2,4-dichlorophenoxyacetic acid, respectively. Embryogenic calluses produced numerous somatic embryos, which then developed into plantlets on 1/2MS without growth regulators. Regenerated plantlets were grown to whole plants in a growth chamber.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-009-0092-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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