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Title 

Plant regeneration from the root-derived embryonic tissues of Rosa hybrida L. cv. Charming via a combined pathway of somatic embryogenesis and organogenesis

Authors 

Suk Weon KimMyung Jin OhJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2009

Citation 

Plant Biotechnology Reports, vol. 3, no. 4, pp. 341-345

Keywords 

OrganogenesisRoot explantsRosa hybrida L.Somatic embryo conversionSomatic embryogenesis

Abstract 

This study describes culture conditions for a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis in root explant cultures of the commercial rose cultivar 'Charming'. Root explants formed white calluses at a frequency of 30% after 6 weeks of culture on Schenk and Hildebrandt (SH) medium supplemented with 11 mg l-1 2,4-dichlorophenoxyacetic acid. After 6 weeks of transfer to SH medium without growth regulators, initial white calluses gave rise to globular somatic embryos at a frequency of 2.8%, which were subsequently dedifferentiated to embryonic tissues. Somatic embryos or embryonic tissues initially derived from root explants did not undergo development beyond cotyledonary stage. To produce adventitious shoots, embryonic tissues were sliced and cultured on SH medium with 0.5 mg l-1 6-benzyladenine. After 4 weeks of culture, 28% of embryonic tissue explants formed adventitious shoots. Regenerated shoots were rooted on half strength SH medium with 0.1 mg l-1 α-naphthalaneacetic acid and subsequently grown to maturity. Root-derived embryonic tissues were proliferated by subculture, while retaining the capacity for shoot production for a few years.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-009-0108-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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