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Title 

Production of fibrinolytic enzyme in plastid-transformed tobacco plants

Authors 

S M KoB H YooJ M LimK H OhJ I LiuSuk Weon KimJang Ryol LiuK S ChoiE S Yoon

Publisher 

Springer Verlag (Germany)

Issue Date 

2009

Citation 

Plant Molecular Biology Reporter, vol. 27, no. 4, pp. 448-453

Keywords 

BiopharmaceuticalChloroplast transformationFibrinolytic enzymeGenetically modified crops

Abstract 

The earthworm fibrinolytic enzyme, which belongs to a group of serine proteases with strong fibrinolytic activity, has been used as an oral drug for prevention and treatment of thrombosis in East Asia. Fibrizyme is a fibrinolytic enzyme isolated from the earthworm Eisenia andrei. Here we report genetic engineering of tobacco plastids with stable integration of the fibrizyme gene into the tobacco chloroplast genome. A plastid transformation vector was constructed by introducing various regulatory elements into fibrizyme cDNA. This vector was delivered by particle bombardment into tobacco leaf explants and plastid-transformed plants were subsequently regenerated into whole plants through several rounds of selection. We confirmed stable integration of the fibrizyme gene into the tobacco plastid genome by PCR and Southern blot analyses. Northern and Western blot analyses revealed that mRNA and protein of recombinant fibrizyme were highly expressed in transformed tobacco plants.

ISSN 

0735-9640

Link 

http://dx.doi.org/10.1007/s11105-009-0113-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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