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Title 

Production of pure β-glucan by Aureobasidium pullulans after pullulan synthetase gene disruption

 

pul 유전자가 제거된 Aureobasidium pullulan에 의한 순수 β-glucan 생산

Authors 

Byeong Gwan KangHeejong YangNack-Shick ChoiKeug Hyun AhnChan Sun ParkByung Dae YoonMin-Soo Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2010

Citation 

Biotechnology Letters, vol. 32, no. 1, pp. 137-142

Keywords 

β-GlucanAureobasidium pullulansHomologous recombinationPullulan

Abstract 

By disruption of the pullulan synthetase gene (pul) of Aureobasidium pullulans IMS822 KCTC11179BP, we constructed a mutant strain, A. pullulans NP1221, which produced a pure β-glucan exopolysaccharide. The mutant NP1221 was white, whereas the wild-type strain produced a black dye. When we compared fermentation kinetics between wide-type and mutant strains, the mutant NP1221 did not produce pullulan. Substrate uptake rate and β-glucan production were similar in both strains. However, the biomass yield of mutant NP1221 was 2.3-fold (9.2 g l-1) greater than that of wild-type.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-009-0127-x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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