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Title 

Activity assay for nisin-like acidic bacteriocins using an optimal pH-conditioned gel matrix

 

pH 조절 gel matrix를 이용한 nisin 유사 baceriocin의 활성 분석

Authors 

Nack-Shick ChoiSung Yeop JungHeejong YangKeug Hyun AhnChan Sun ParkCha Young KimJoong Su KimByung Dae YoonMin-Soo Kim

Publisher 

Elsevier

Issue Date 

2010

Citation 

Analytical Biochemistry, vol. 397, no. 2, pp. 259-261

Abstract 

A new zymography for detecting nisin-like acidic bacteriocins was developed using a tricine-sodium dodecyl sulfate (SDS) gel and an acidic gel matrix (pH 4.0). After electrophoresis, proteins in the tricine gel were electrotransferred to an optimal pH-conditioned gel matrix (OP-CGM). The OP-CGM was overlaid with indicator cells (Bacillus cereus) embedded in nutrient broth soft agar (0.8%, w/v). Antibacterial activity shown as a growth inhibition using B. cereus was detected at approximately 3.8 kDa. Because nisin is unstable in buffers at pH values over 6.0, the common electrophoretic systems, SDS-polyacrylamide gel electrophoresis and tricine gel, are not suitable for detection of nisin-like acidic bacteriocins.

ISSN 

0003-2697

Link 

http://dx.doi.org/10.1016/j.ab.2009.09.046

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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