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Title 

Increase of RhoB in γ-radiation-induced apoptosis is regulated by c-Jun N-terminal kinase in Jurkat T cells

Authors 

C H KimMi Sun WonJ H ChoiJi Won AhnBo Kyung KimK B SongC M KangKyung Sook Chung

Publisher 

Elsevier

Issue Date 

2010

Citation 

Biochemical and Biophysical Research Communications, vol. 391, no. 2, pp. 1182-1186

Keywords 

γ-RadiationApoptosisJNKJurkatRhoB

Abstract 

The Ras-related small GTP-binding protein RhoB is known to be a pro-apoptotic protein and immediate-early inducible by genotoxic stresses. In addition, JNK activation is known to function in γ-radiation-induced apoptosis. However, it is unclear how JNK activation and γ-radiation-dependent RhoB induction are related. Here we verified the relationship between JNK activation and RhoB induction. RhoB induction by γ-radiation occurred at the transcriptional level and transcriptional activation of RhoB was concomitant with an increase in RhoB protein. γ-Radiation-induced RhoB expression was markedly attenuated by pretreatment with a JNK-specific inhibitor, SP600125, but not by a p38 MAPK inhibitor, SB203580. Inhibition of JNK caused a decrease in early apoptotic cell death that correlated with RhoB expression. However, PI3K inhibition had no significant effects, indicating that the AKT survival pathway was not involved. The siRNA knockdown of JNK resulted in a decrease in RhoB expression and the siRNA knockdown of RhoB restored cell growth even in the γ-irradiated cells. These results suggest that RhoB regulation involves the JNK pathway and contributes to the early apoptotic response of Jurkat T cells to γ-radiation.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2009.12.012

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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