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Title 

Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100

Authors 

Kwon HwangBoSu Hyun SonJong Suk LeeSung Ran MinS M KoJang Ryol LiuD ChoiWon Joong Chung

Publisher 

Springer Verlag (Germany)

Issue Date 

2010

Citation 

Plant Biotechnology Reports, vol. 4, no. 1, pp. 49-52

Keywords 

Chelex 100DNA extraction methodPCRPlant materialTransgenes

Abstract 

A DNA extraction method using Chelex 100 is widely used for bacteria, Chlamydomonas, and animal cell lines, but only rarely for plant materials due to the need for additional time-consuming and tedious steps. We have modified the Chelex 100 protocol and successfully developed a rapid and simple method of DNA extraction for efficient PCR-based detection of transgenes from a variety of transgenic plant and algal species. Our protocol consists of homogenizing plant tissue with a pestle, boiling the homogenized tissue in a microfuge tube with 5% Chelex 100 for 5 min, and centrifuging the boiled mixture. The supernatant, which is used for PCR analysis, was able to successfully amplify transgenes in transgenic tobacco, tomato, potato, Arabidopsis, rice, strawberry, Spirodela polyrhiza, Chlamydomonas, and Porphyra tenera. The entire DNA extraction procedure requires <15 min and is therefore comparable to that used for bacteria, Chlamydomonas, and animal cell lines.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-009-0117-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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