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Title 

Development of one-step real-time reverse transcription polymerase chain reaction assays for rapid detection of porcine group C rotaviruses

Authors 

Y H ChunY J JeongS I ParkM HosmilloD J ShinH J KwonS Y KangS K WooM I KangSu-Jin ParkK O Cho

Publisher 

American Association of Veterinary Laboratory Diagnosticians

Issue Date 

2010

Citation 

Journal of Veterinary Diagnostic Investigation, vol. 22, no. 1, pp. 74-77

Keywords 

DiagnosisPorcine group c rotavirusReal-time reverse transcription polymerase chain reactionSybr greenTaqman

Abstract 

Although the widespread occurrence of porcine group C rotaviruses (GCRV) is assumed, precise prevalence remains largely unknown because of the absence of reliable, specific, and rapid diagnostic methods. To detect and quantify porcine GCRV, the authors evaluated and optimized SYBR Green and TaqMan real-time reverse transcription polymerase chain reaction (RT-PCR) assays and applied them to 108 piglet fecal samples. Using serially diluted standard RNA transcripts of porcine GCRV VP6 gene, both SYBR Green and TaqMan real-time RT-PCR assays detected as few as 1 3 101 genome copies/ml (correlation coefficiency .0.99), whereas conventional RT-PCR detected 1.0 3 103 copies/ml. In addition, the conventional assay detected porcine GCRV in 24% (26/108) of fecal samples, whereas the detection rates of both SYBR Green and TaqMan assays were 72% (78 of 108) and 64% (70 of 108), respectively. The current study indicated that both real-time RT-PCR assays were reliable, specific, and rapid methods for the detection of porcine GCRV in porcine fecal samples.

URI 

https://doi.org/10.1177/104063871002200113

ISSN 

1040-6387

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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