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Title 

TSPYL5 is involved in cell growth and the resistance to radiation in A549 cells via the regulation of p21WAF1/Cip1 and PTEN/AKT pathway

Authors 

E J KimS Y LeeT R KimS I ChoiEun Wie ChoK C KimI G Kim

Publisher 

Elsevier

Issue Date 

2010

Citation 

Biochemical and Biophysical Research Communications, vol. 392, no. 3, pp. 448-453

Keywords 

γ-RadiationAKTPTENTSPYL5

Abstract 

TSPYL5, encoding testis-specific Y-like protein, has been postulated to be a tumor suppressor gene, and its hypermethylation is often associated with human disease, especially cancer. In this study, we report that the TSPYL5 gene was less methylated (30%) in A549 lung adenocarcinoma cells, which are relatively resistant to γ-radiation, than in H460 lung cancer cells, in which the TSPYL5 gene was hypermethylated (95%); thus, the expression level of TSPYL5 is much higher in A549 cells than in H460 cells. We showed that TSPYL5 suppression with silencing RNA in A549 cells up-regulated cellular PTEN, followed by down-regulation of AKT activation. Therefore, blockage of TSPYL5 sensitized A549 cells to cytotoxic agents such as γ-radiation. In addition, TSPYL5 suppression also showed an increased level of p21WAF1/Cip1 and subsequently induced inhibition of cell growth in A549 cells. The overexpression of TSPYL5 in H460 cells showed the opposite effects. This study provides the first demonstration that TSPYL5 modulates cell growth and sensitization of cells to the detrimental effects of damaging agents via regulation of p21WAF1/Cip1 and PTEN/AKT pathway.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2010.01.045

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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