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Title 

Exogenous glutamate inhibits the root growth and increases the glutamine content in Arabidopsis thaliana

Authors 

T H KimE C KimSuk Weon KimH S LeeD W Choi

Publisher 

The Botanical Society of Korea

Issue Date 

2010

Citation 

Journal of Plant Biology, vol. 53, no. 1, pp. 45-51

Keywords 

ArabidopsisGlutamateGlutaminepHRoot growth

Abstract 

Previously, our work with ginseng hairy root shows that the tissue of low-branching and slow-growing phenotype contains high level of glutamine. In order to check if the high glutamine concentration inhibits the root growth, we applied exogenous glutamine or glutamate into growth medium and check the root growth of Arabidopsis. While glutamine did not affect root growth, over 0. 1 mM glutamate inhibited severe root growth. However, when the amino acid solution was adjusted to pH 5. 7 and added into medium, Arabidopsis seedlings show normal growth pattern on medium containing glutamate or aspartate. These results demonstrated that inhibition of the root growth by high concentration of exogenous glutamate was a result of the low pH toxicity caused by acidic amino acid, although low concentration (0. 05 mM) of glutamate has an inhibitory effect on the primary root growth. The application of exogenous glutamine or glutamate increases glutamine concentration within root tissue about 3- to 4-fold. However, concentration of glutamate is not significantly increased. The KO mutant on each of the Gln1_1, Gln1_2, or Glu2 gene was little effective on the root growth. These results indicate that high concentration of endogenous glutamine observed in root tissue does not affect root growth.

ISSN 

1226-9239

Link 

http://dx.doi.org/10.1007/s12374-009-9084-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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